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2018 ACA CryoEM Workshop

The Cryo-EM Workshop: A Guide to High Resolution Structure Determination was held on July 21, 2018 as part of the American Crystallographic Association Annual Meeting in Toronto, Canada. Co-organized by Lori Passmore (MRC-LMB) and Wah Chiu (Stanford U), and sponsored by Douglas Instruments, Gatan, and ThermoFisher Scientific, the one-day workshop attracted over 130 attendees.


workshop image

Cryo-EM has become increasingly popular among structural biologists and is now the method of choice for structure determination of proteins larger than ~200 kDa. It has been particularly successful for proteins that are difficult to crystallize, including membrane proteins, large assemblies and multi-protein complexes. The workshop provided a detailed overview of cryo-EM specimen preparation, image processing and building/refinement of atomic models, with focus on high-resolution single particle cryo-EM, and several hands-on tutorials. Materials from the workshop can be accessed through the links below.


Go to this public Box folder or this public DropBox folder to access talk pdfs and workshop zip files.


Slides/Handouts

Instructor Topic
Wah Chiu Welcome and Introduction to Cryo-EM
Lori Passmore Specimen Preparation for Cryo-EM
Chris Russo Specimen Preparation, Data Collection and Radiation Damage || Russo&Henderson2018
John Rubinstein Image Processing — Intro, Background, Implementation
Marcus Brubaker Image Processing — Bayesian Methods and Optimization
Ali Punjani CryoSparc Image Processing Tutorial
Tom Terwilliger Phenix tools for Cryo-EM
Matthew Baker Model Building in Cryo-EM || Pathwalking Tutorial
Corey Hryc Model Optimization Tutorial || zipfile
Corey Hryc Model Validation Tutorial || zipfile

Tutorial Software

These software packages are available on Mac, Linux and Windows.
  • UCSF Chimera is used to visualize/manipulate both map and model.
  • Phenix (command line) is used to optimize the molecular model with respect to the experimental data.
  • EMAN2 is used for Pathwalking as well as validation routines such as map/model FSC.
  • COOT is used for model refinement in the Pathwalker Tutorial.

updated 24 July 2024


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National Institutes of Health
National Institute of General Medical Sciences

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